Hampton Crystal Screen Solutions

Tube #

SALT

BUFFER

Precipitant

Miniscreen

Tube #

1

0.02M Calcium Chloride

0.1M Na Acetate pH 4.6

30% w/v 2-methyl-2,4-pentanediol

Y

1

2

None

None

0.4M K,Na Tartrate tetrahydrate

2

3

None

None

0.4M Ammonium dihydrogen phosphate

3

4

None

0.1M Tris-HCl pH 8.5

2.0M Ammonium Sulfate

Y

4

5

0.2M tri-sodium citrate

0.1M Na HEPES pH 7.5

30% w/v 2-methyl-2,4-pentanediol

5

6

0.2M Magnesium chloride

0.1M Tris-HCl pH 8.5

30% w/v PEG 4000

6

7

None

0.1M Na Cacodylate pH 6.5

1.4M Sodium acetate trihydrate

7

8

0.2M tri-sodium citrate

0.1M Na Cacodylate pH 6.5

30% v/v 2-propanol

8

9

0.2M Ammonium acetate

0.1M Na Citrate pH 5.6

30% w/v PEG 4000

Y

9

10

0.2M Ammonium acetate

0.1M Na Acetate pH 4.6

30% w/v PEG 4000

Y

10

11

None

0.1M Na Citrate pH 5.6

1.0M Ammonium dihydrogen phosphate

11

12

0.2M Magnesium chloride

0.1M Na HEPES pH 7.5

30% v/v 2-propanol

12

13

0.2M tri-sodium citrate

0.1M Tris-HCl pH 8.5

30% v/v PEG 400

13

14

0.2M Calcium Chloride

0.1M Na HEPES pH 7.5

28% v/v PEG 400

Y (best)

14

15

0.2M Ammonium acetate

0.1M Na Cacodylate pH 6.5

30% w/v PEG 8000

15

16

None

0.1M Na HEPES pH 7.5

1.5M Lithium sulfate monohydrate

Y

16

17

0.2M Lithium sulfate

0.1M Tris-HCl pH 8.5

30% w/v PEG 4000

Y (2nd best)

17

18

0.2M Magnesium acetate

0.1M Na Cacodylate pH 6.5

20% w/v PEG 8000

18

19

0.2M Ammonium acetate

0.1M Tris-HCl pH 8.5

30% v/v 2-propanol

19

20

0.2M Ammonium sulfate

0.1M Na Acetate pH 4.6

25% w/v PEG 4000

20

21

0.2M Magnesium acetate

0.1M Na Cacodylate pH 6.5

30% v/v 2-methyl-2,4-pentanediol

Y

21

22

0.2M Sodium acetate

0.1M Tris-HCl pH 8.5

30% w/v PEG 4000

Y

22

23

0.2M Magnesium chloride

0.1M Na HEPES pH 7.5

30% v/v PEG 400

23

24

0.2M Calcium Chloride

0.1M Na Acetate pH 4.6

20% v/v 2-propanol

Y

24

25

None

0.1M Imidazole pH 6.5

1.0M Sodium acetate trihydrate

25

26

0.2M Ammonium acetate

0.1M Na Citrate pH 5.6

30% v/v 2-methyl-2,4-pentanediol

26

27

0.2M tri-sodium citrate

0.1M Na HEPES pH 7.5

20% v/v 2-propanol

27

28

0.2M Sodium acetate

0.1M Na Cacodylate pH 6.5

30% w/v PEG 8000

28

29

None

0.1M Na HEPES pH 7.5

0.8M K,Na Tartrate

29

30

0.2M Ammonium acetate

None

30% w/v PEG 8000

30

31

0.2M Ammonium acetate

None

30% w/v PEG 4000

Y

31

32

None

None

2.0M Ammonium Sulfate

32

33

None

None

4.0M Sodium formate

33

34

None

0.1M Na Acetate pH 4.6

2.0M Sodium formate

Y

34

35

None

0.1M Na HEPES pH 7.5

1.6M K,Na Tartrate

Y

35

36

None

0.1M Tris-HCl pH 8.5

8% w/v PEG 8000

Y

36

37

None

0.1M Na Acetate pH 4.6

8% w/v PEG 4000

37

38

None

0.1M Na HEPES pH 7.5

1.4M Sodium citrate dihydrate

38

39

None

0.1M Na HEPES pH 7.5

2% v/v PEG 400 & 2.0M Ammonium Sulfate

39

40

None

0.1M Na Citrate pH 5.6

20% v/v 2-propanol & 20% w/v PEG 4000

40

41

None

0.1M Na HEPES pH 7.5

10% v/v 2-propanol & 20% w/v PEG 4000

41

42

0.05 K-phosphate monobasic

None

20% w/v PEG 8000

Y

42

43

None

None

30% w/v PEG 1500

43

44

None

None

0.2M Magnesium formate

Y

44

45

0.2M Zinc acetate

0.1M Na Cacodylate pH 6.5

18% w/v PEG 8000

45

46

0.2M Calcium acetate

0.1M Na Cacodylate pH 6.5

18% w/v PEG 8000

46

47

None

0.1M Na Acetate pH 4.6

2.0M Ammonium Sulfate

Y

47

48

None

0.1M Tris-HCl pH 8.5

2.0M Ammonium dihydrogen phosphate

48

49

1.0M Lithium Sulfate

None

2% w/v PEG 8000

Y

49

50

0.5M Lithium Sulfate

None

15% w/v PEG 8000

50